An experimental assay of the interactions of amino acids from orthologous sequences shaping a complex fitness landscape

by Victoria O. Pokusaeva, Dinara R. Usmanova, Ekaterina V. Putintseva, Lorena Espinar, Karen S. Sarkisyan, Alexander S. Mishin, Natalya S. Bogatyreva, Dmitry N. Ivankov, Arseniy V. Akopyan, Sergey Ya. Avvakumov, Inna S. Povolotskaya, Guillaume J. Filion, Lucas B. Carey, Fyodor A. Kondrashov

Characterizing the fitness landscape, a representation of fitness for a large set of genotypes, is key to understanding how genetic information is interpreted to create functional organisms. Here we determined the evolutionarily-relevant segment of the fitness landscape of His3, a gene coding for an enzyme in the histidine synthesis pathway, focusing on combinations of amino acid states found at orthologous sites of extant species. Just 15% of amino acids found in yeast His3 orthologues were always neutral while the impact on fitness of the remaining 85% depended on the genetic background. Furthermore, at 67% of sites, amino acid replacements were under sign epistasis, having both strongly positive and negative effect in different genetic backgrounds. 46% of sites were under reciprocal sign epistasis. The fitness impact of amino acid replacements was influenced by only a few genetic backgrounds but involved interaction of multiple sites, shaping a rugged fitness landscape in which many of the shortest paths between highly fit genotypes are inaccessible.

Tratto da: www.plos.org
Note sul Copyright: Articles and accompanying materials published by PLOS on the PLOS Sites, unless otherwise indicated, are licensed by the respective authors of such articles for use and distribution by you subject to citation of the original source in accordance with the Creative Commons Attribution (CC BY) license.