Make us Happy! Subscribe our YouTube Channel. We don't accept donations, and this is the best way to sustain our work.
Scarica subito gratis il file PDF del testo della trasmissione

Phylogenetic analysis of migration, differentiation, and class switching in B cells

by Kenneth B. Hoehn, Oliver G. Pybus, Steven H. Kleinstein

B cells undergo rapid mutation and selection for antibody binding affinity when producing antibodies capable of neutralizing pathogens. This evolutionary process can be intermixed with migration between tissues, differentiation between cellular subsets, and switching between functional isotypes. B cell receptor (BCR) sequence data has the potential to elucidate important information about these processes. However, there is currently no robust, generalizable framework for making such inferences from BCR sequence data. To address this, we develop three parsimony-based summary statistics to characterize migration, differentiation, and isotype switching along B cell phylogenetic trees. We use simulations to demonstrate the effectiveness of this approach. We then use this framework to infer patterns of cellular differentiation and isotype switching from high throughput BCR sequence datasets obtained from patients in a study of HIV infection and a study of food allergy. These methods are implemented in the R package dowser, available at

Read More

Unless otherwise indicated, articles and accompanying materials published by PLOS on the PLOS Sites, including peer reviews, are licensed by the respective authors for use and distribution by you subject to citation of the original source in accordance with the Creative Commons Attribution (CC BY) license.

Lascia un commento

Il tuo indirizzo email non sarà pubblicato.

I nostri audiolibri su Audible